EFECTO DE LA CAFEÍNA Y LA RHUS TRILOBATA SOBRE EL DESARROLLO DE COMPETENCIA DE OVOCITOS BOVINOS MADURADOS IN VITRO

RODRÍGUEZ BORBÓN, ADRIANA (2017) EFECTO DE LA CAFEÍNA Y LA RHUS TRILOBATA SOBRE EL DESARROLLO DE COMPETENCIA DE OVOCITOS BOVINOS MADURADOS IN VITRO. Maestría thesis, UNIVERSIDAD AUTONOMA DE CHIHUAHUA.

Vista Previa

Text Tesis.pdf Download (971Kb) | Vista Previa

Resumen

The objective was to evaluate the effect of caffeine (CAFN) and Rhus trilobata (RT) on oocyte maturation. Cumulus oocytes complexes (COC’s) were aspired from cows ovaries provided from a slaughterhouse, cultivated for 23 h, and distributed in four groups: CONTROL, CAFN 10 mM, RT 3 µg / mL, and CAFN+RT. Compaction of COC’s, polar body (CP) extrusion, ROS – GSH levels, development of oocytes after parthenogenesis, and In vitro fertilization rate were evaluated. The COC's were maintained compacted (64.30 %) and meiotic resumption was kept inhibited by action of CAFN; only just a low percentage extruded CP at the end of the maturation (5.77 %). The RT improved nuclear maturation (CP; 42.81 %) in comparison with the rest of the groups and kept the COC’s compacted. The CAFN increased (P < 0.05) ROS contents (1.6539 UAF) in comparison with the RT (1.1805 UAF) and CAFN+RT (1.0815 UAF). GSH concentration was similar (P > 0.05) among treatments. The celular division of the oocytes artificially activated or fertilized was similar (P > 0.05) among groups. These results suggest that the RT promotes the ability of develop competition of the oocyte whereas the CAFN acted as a potent meiosis inhibitor, besides it decreases oocyte viability and ability to develop competition of the oocyte.

Actions (login required)

Ver Objeto