PARÁMETROS DE FERMENTACIÓN RUMINAL Y DINÁMICA DE LA MICROBIOTA RUMINAL IN VITRO BAJO LA INFLUENCIA DE TIMOL Y CARVACROL

CASTAÑEDA CORREA, ALEJANDRO (2016) PARÁMETROS DE FERMENTACIÓN RUMINAL Y DINÁMICA DE LA MICROBIOTA RUMINAL IN VITRO BAJO LA INFLUENCIA DE TIMOL Y CARVACROL. Maestría thesis, UNIVERSIDAD AUTONOMA DE CHIHUAHUA.

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Two essential oils (EO), thymol (T) and carvacrol (C), were used in six ratios (100: 00, 80:20, 60:40, 40:60, 20:80 and 00:100) of combination and in a dose of 0.2 g·L-1 of media to evaluate its effect on total gas production (TGP), methane (CH4), in vitro dry matter degradability (IVDMD), and population dynamics of methanogenic and total bacteria under in vitro conditions. The incubation was performed using bovine ruminal media for 24 h. After incubation, PTG, CH4 and IVDMD were determined. Then, DNA was extracted from ruminal microorganisms and PCR-DGGE was performed. Finally, bacterial population dynamics from rumen was analyzed. The final results of these variables were diverted over control to obtain the percentage of change due to treatment. In general, treatment with the highest concentration of T caused a higher rate of change in the production of CH4, and a tendency to decrease linearly (P < 0.05) as the concentration of T was reduced by the EO. It was observed that the population of total bacteria showed a slight variation in structure (DSC = 88.1 %), which indicates the similarity between a taken sample relative to a reference sample. No effects (P > 0.05) on PTG, DIVMS and population of methanogenic bacteria (DSC = 96.1 %) were observed. The T showed the greatest effect on CH4 production, and slightly modifying the populations of total bacteria, but with the advantage of not affecting TGP, IVDMD and populations of methanogenic bacteria.

Tipo de Documento: Tesis (Maestría)
Palabras Clave: Essential oils, thymol, carvacrol, methane and fermentation parameters
Clasificación temática: Q Science > Q Science (General)
Usuario Remitente: Admin Administrador del Respositorio
Depositado: 21 Jun 2018 16:59
Ultima Modificación: 21 Jun 2018 16:59
URI: http://repositorio.uach.mx/id/eprint/167

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Universidad Autonoma de Chihuahua