Inducción de variabilidad mediante irradiación gamma en pasto africano (Eragrostis Iehmanniana) y garrapata (Eragrostis superba)

Álvarez Holguín, Alan (2018) Inducción de variabilidad mediante irradiación gamma en pasto africano (Eragrostis Iehmanniana) y garrapata (Eragrostis superba). Doctorado thesis, Universidad Autónoma de Chihuahua.

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The lack of genetic diversity on alien plant species hinders its improvement and use. Thus, the mutagenesis is a viable breeding alternative on these species, since it has proven to be an effective technique inducing genetic variability. Therefore, the objective was to induce variability on Wilman (Eragrostis superba) and Lehmann (Eragrostis lehmanniana) love grasses through gamma radiation, for breeding purposes. For this, four studies under laboratory and greenhouse conditions were performed. The first and second studies were carried out to determine the optimal gamma irradiation dose for the Wilman and Lehmann love grasses breeding, respectively. This was determined by the median lethal (LD50) and growth reduction (GR50) doses. In the third study, the morphological, nutritional, stomatal and molecular diversity induced through gamma irradiation on Wilman lovegrass were characterized. Finally, the fourth study was carried out to characterize the morphological and nutritional variability induced by gamma irradiation on Lehmann lovegrass. The gamma radiation generated phenotypic and genetic variability on Wilman lovegrass. This, allowed to select the first generation of mutants with more (P<0.05) protein and less (P<0.05) lignin, than the control genotype. These mutants presented differences (P<0.05) respecting the control plants in the stomatal density and area, which has repercussions on the water stress tolerance. In addition, they showed significant genetic distances to the control genotype (from 0.25 to 0.42, DICE coefficient). As in Wilman lovegrass, the gamma irradiation generated morphological and nutritional variability on Lehmann lovegrass, which enabled to select the first generation of mutants. Three mutants (200-6, 300-7 y 1400-10) with less lignin and more protein (P<0.05) were selected. Therefore, it is recommended to evaluate the selected mutant’s progeny, until the selected characters will be fixed. In case of being able to fix these characteristics, new genetic material of agronomic and ecological interest will be available, which could be used rehabilitating degraded grasslands.

Tipo de Documento: Tesis (Doctorado)
Palabras Clave: Mutagenesis, gamma rays, plant breeding, grasslands rehabilitation
Clasificación temática: S Agriculture > SB Plant culture
Usuario Remitente: Admin Administrador del Respositorio
Depositado: 26 Jun 2019 15:51
Ultima Modificación: 26 Jun 2019 15:51
URI: http://repositorio.uach.mx/id/eprint/197

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Universidad Autonoma de Chihuahua